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RE: Methods, Methods, Methods (My Research, Vol. 5)

in #science6 years ago

Nice job! A few things to remember:

  • Run to red
  • You can check your gel tray for little bubbles to make sure stuff is hooked up OK
  • EtBR is less toxic than we thought, but if EH&S is giving you issues, there are alternative dyes (GelRed)

PCR tips:
https://theupturnedmicroscope.com/tag/pcr/
https://goo.gl/images/2UQYW3

Sort:  
  • We have everything color-labeled because of this
  • Never leave the lab without checking for bubbles
  • I don't exactly have a choice what to use ^^